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Product Info Summary
| SKU: | A01036-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KCNH1 Antibody Picoband™
SKU/Catalog Number
A01036-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KCNH1 Antibody Picoband™ catalog # A01036-4. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KCNH1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01036-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human KCNH1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01036-4 is reactive to KCNH1 in Human, Rat
Applications
A01036-4 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
111.423kDa
Background of KCNH1
Potassium voltage-gated channel subfamily H member 1 is a protein that in humans is encoded by the KCNH1 gene. Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KCNH1 using anti-KCNH1 antibody (A01036-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH1 antigen affinity purified polyclonal antibody (Catalog # A01036-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH1 at approximately 111 kDa. The expected band size for KCNH1 is at 111 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KCNH1 (Source: Uniprot.org, NCBI)
Gene Name
KCNH1
Full Name
Potassium voltage-gated channel subfamily H member 1
Weight
111.423kDa
Superfamily
potassium channel family
Alternative Names
EAG channel 1; EAG1; EAGeag1; Ether-a-go-go potassium channel 1; ether-a-go-go, Drosophila, homolog of; hEAG1; h-eageag; Kv10.1; MGC142269; potassium channel, voltage-gated, subfamily H, member 1; potassium voltage-gated channel subfamily H member 1; potassium voltage-gated channel, subfamily H (eag-related), member 1; Voltage-gated potassium channel subunit Kv10.1 KCNH1 EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1 potassium voltage-gated channel subfamily H member 1 potassium voltage-gated channel subfamily H member 1|EAG channel 1|ether-a-go-go 1|ether-a-go-go potassium channel 1|ether-a-go-go, Drosophila, homolog of|potassium channel, voltage gated eag related subfamily H, member 1|potassium voltage-gated channel, subfamily H (eag-related), member 1|voltage-gated potassium channel subunit Kv10.1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNH1, check out the KCNH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNH1 Antibody Picoband™ (A01036-4)
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No publications found for A01036-4
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